How Ozone Works

Ozone has 3,125 times stronger disinfecting power on bacteria and virus compared with chlorine and ozonated water has 50+ times stronger than chemical bactericidal agent such as chlorine bleach.

Biotek 2 ppm ozonated water can kills 99.999% tested bacteria within 5 to 15 seconds as shown in SGS report. The required concentration is far less than chemical bactericide such as chlorine that requires 100+ ppm of chlorine for normal disinfection and up to 1,000 ppm for medical disinfection. Thus, using Biotek Ozonated water can prevents bactericidal chemical residue problems and have advantage of cost saving on water for rinsing and prevent re-contaminated of disinfected materials by bacteria containing water.

Instant Bacteria Killer


Bacterial Disinfection Mechanism

All bacteria and viruses are composed of carbohydrate, protein, RNA and DNA. For structure of protozoa and bacteria, all cell organs are surrounded by cell wall and cell membrane Ozone molecules in ozonated water has high oxidation potential and it oxidizes cell components of the bacterial cell envelope which is a consequence of cell wall penetration. When ozone has entered the cell, it oxidizes all essential components (enzymes, proteins, DNA, RNA). This mechanism differs from that of halogens (such as chlorine), which are usually applied. Chlorine is known to penetrate cells by diffusion. Within the cell, chlorine affects several enzyme types.


Scientific journals about ozone killing bacteria and virus

Ozone’s bactericidal effects centers on disruption of envelope integrity through peroxidation of phospholipids and lipoproteins. There is evidence for interaction with proteins as well[1]. In one study[2] exploring the effect of ozone on E. coli, evidence was found for ozone’s penetration of the cell membrane, reacting with cytoplasmic substances and converting the closed circular plasmid DNA to open circular DNA, which would presumably lessen the efficiency of bacterial proliferation. It is notable that higher organisms have enzymatic mechanisms to restabilize disrupted DNA and RNA, which could provide a partial explanation for why, in clinical treatment with ozone at doses prescribed, ozone appears to be toxic to infecting organisms and not to the patient.[3]

For anti-viral ability of ozone In one study,[4] polio virus 1 was exposed to 2.1 ppm. of ozone at pH 7.2. After 30 seconds 99% of the viruses were inactivated (lost their ability to replicate within host cells). Analysis of viral components showed damage to polypeptide chains and envelope proteins, which could result in attachment capability compromise, and breakage of the single-stranded RNA into two parts, producing replicating dysfunction at its root level. Other researchers[5] in similar experiments concluded that in ozonation, it is the viral capsid which sustains damage.


[1] Mudd JB, Leavitt R, Ongun A, McManus T: Reaction of ozone with amino acids and proteins. Atmos Environ 1969;3:669-682.

[2] Ishizaki K, Sawadaishi D, Miura K, Shinriki N: Effect of ozone on plasmid DNA of Escheria coli in situ. Water Res 1987;21(7):823-828.

[3] Cech T: RNA as an enzyme. Scientific American 1986 Nov;255(5):64-76.

[4] Roy D, Wong PK, Engelbrecht RS, Chian ES: Mechanism of enteroviral inactivation by ozone. Appl Envir Microbiol 1981;41:718-723.

[5] Riesser V, Perrich J, Silver B, McCammon J: Possible mechanimsm of poliovirus inactivation by ozone, in Forum on Ozone Disinfection.

Proceedings of the International Ozone Institute. Syracuse, NY, 1977; pp. 186-192.



Bacteria are microscopically small, single-cell creatures having a primitive structure. The bacteria body is sealed by a relatively solid-cell membrane. Ozone interferes with the metabolism of bacterium-cells, most likely through inhibiting and blocking the operation of the enzymatic control system. A sufficient amount of ozone breaks through the cell membrane, and this leads to the destruction of the bacteria. Viruses are small, independent particles, built of crystals and macromolecules. Unlike bacteria, they multiply only within the host cell. They transform protein of the host cell into proteins of their own. Ozone destroys viruses by diffusing through the protein coat into the nucleic acid core, resulting in damage of the viral RNA. At higher concentrations, ozone destroys the capsid, or exterior protein shell by oxidation so DNA (deoxyribonucleic acid), or RNA (ribonucleic acid) structures of the microorganism are affected.

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